MicroTime 200 STED, PicoQuant 

The MicroTime 200 STED is a time-resolved confocal microscope with single molecule sensitivity and super-resolution capabilities.

Laser Excitation Modules:

STED module contains 766 nm (STED laser), 595 nm and 640 nm lasers;
VIS module contains 440 nm, 485 nm and 532 nm lasers.

Main Applications:

* Stimulated Emission Depletion Microscopy (STED), optical resolution below 50 nm
* Single Molecule Imaging
* Fluorescence Lifetime Imaging (FLIM)
* Fluorescence Correlation Spectroscopy (FCS)
* Lifetime-based Forster Resonance Energy Transfer (FLIM-FRET)
* Pulsed Interleaved Excitation (PIE)

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LSM 710 ConfoCor 3, Zeiss

LSM 710 ConfoCor 3 is a flexible and modular confocal laser scanning microscope for high end fluorescence imaging with highest sensitivity, resolution and speed and FCS spectroscopy. 405 nm, 488 nm 543 nm and 632 nm CW lasers are employed for fluorescence excitation. The system contains 2 PMTs and 2 APDs for fluorescence detection, and 1 PMT for transmission measurements.

Main Applications:

* Fluorescence correlation (FCS) and crosscorrelation (FCCS) spectroscopy
* Quantitative Imaging with single molecule sensitivity
* Spectral Imaging
* Fluorescence recovery after photobleaching (FRAP)

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AxioObserver, inverted microscope, Zeiss

The AxioObserver employs LEDs (Colibri, Zeiss) as an excitation source of fluorescence. Currently, 365 nm, 420 nm, 470 nm and 590 nm LEDs are installed. DefiniteFocus monitors and reliably compensates focus drifts with a closed loop system. The microscope equipped with an incubation chamber for temperature control.

Main Applications:

Long term bright field and fluorescence live cell imaging

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Wide-Field Total Iinternal Reflection Fluorescence (TIRF) microscope

The TIRF microscope is built around an Olympus IX81 inverted microscope and equipped with a Hamamatsu EM-CCD camera allowing single-molecule detection. ZDC (Z Drift Compensation) module monitors and reliably compensates focus drifts. As fluorescence activation and excitation sources the following CW- laser lines are used: 405 nm, 488 nm, 514 nm, 561 nm and 640 nm.

Main Applications:

* Single-molecule imaging
* Super resolution microscopy
* Time lapse imaging

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